Comparison of the in vitro activities of ofloxacin and tetracycline against Chlamydia trachomatis as assessed by indirect immunofluorescence.
نویسندگان
چکیده
The in vitro activity of ofloxacin (DL8280), a new pyridone carboxylic acid, against Chlamydia trachomatis serotype E was assessed by growth of chlamydia in cycloheximide-treated McCoy cells. Chlamydia-infected monolayers were exposed to the drug for 48 and 96 h and for 48 h followed by 48 h of further incubation after withdrawal of ofloxacin. Inclusions were visualized by indirect immunofluorescence, and the effect of the drug was assessed both by inclusion development and by the number of inclusion-forming units generated in the monolayers. The action of ofloxacin was completely lethal when monolayers were exposed to a concentration of 1 microgram/ml throughout one chlamydial developmental cycle. In contrast, tetracycline, when examined by similar techniques, inhibited inclusion development at a concentration of 0.3 micrograms/ml, but was only lethal at 2.4 micrograms/ml. Ofloxacin may prove to be a useful new antichlamydial agent.
منابع مشابه
In vitro activities of ofloxacin and four other new quinoline-carboxylic acids against Chlamydia trachomatis.
The in vitro activities of five new quinoline-carboxylic acids against 2 reference strains and 45 clinical isolates of Chlamydia trachomatis of genital origin were compared with the activities of minocycline and doxycycline. Ofloxacin was the third most active agent (after the two tetracyclines), followed by ciprofloxacin, NY-198, and AM-833.
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Sparfloxacin was more potent than other quinolones (tosufloxacin, lomefloxacin, ciprofloxacin, ofloxacin, fleroxacin, enoxacin, and norfloxacin) and as potent as minocycline and doxycycline in activity against Chlamydia trachomatis in vitro and in vivo. Sparfloxacin was more bactericidal than minocycline against C. trachomatis D/UW-3/Cx.
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The in vitro activities of four quinoline carboxylic acids against 48 strains of Neisseria gonorrhoeae, 10 of Chlamydia trachomatis, and 32 of Ureaplasma urealyticum were compared. Ciprofloxacin was the most active against N. gonorrhoeae and C. trachomatis but had poor bactericidal activity against U. urealyticum, whereas ofloxacin showed the most bactericidal activity against U. urealyticum bu...
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Background and Aim: Chlamydia trachomatis is an obligate intracellular parasite responsible for ocular and genital infections in human. Chlamydia trachomatis is the most common cause of chronic follicular conjunctivitis and is also responsible for 20% of acute conjunctivitis cases. As a rapid diagnosis is important in the reducing the long-term squeal of the diseases, the objective of this stud...
متن کاملThe Prevalence of Chlamydia trachomatis Infection in Women Attending Health Clinics in Shiraz, Islamic Republic of Iran
Background Chlamydia trachomatis (CT) is a common cause of sexually transmitted infections (STI) and a prevalent microorganism found in the vaginal discharge of sexually active women. The infection usually has no symptoms; although it may cause chronic complications such as pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain, tubal infertility and cervical cancer. This stu...
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عنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 26 1 شماره
صفحات -
تاریخ انتشار 1984